Category:Science & Research · Sub-category: biology-medicine · Last updated:
Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.
About this skill (catalog notes)
bio-genome-engineering-off-target-prediction includes at least one code block. At roughly 2,990 words the SKILL.md is on the longer end of the catalog distribution.
How bio-genome-engineering-off-target-prediction fits the catalog
bio-genome-engineering-off-target-prediction sits in the Science & Research category under the biology-medicine sub-topic in the ClaudSkills catalog. There are 10 related skills indexed alongside it; comparing a few before installing usually reveals which fits your workflow best.
These notes are auto-generated from features detected in the SKILL.md file and from this catalog's structure — they aren't part of the source repository.
What this skill does
bio-genome-engineering-off-target-prediction is a community-contributed Claude Code skill in the biology-medicine sub-category. It ships as a SKILL.md file that Claude Code auto-discovers under ~/.claude/skills/bio-genome-engineering-off-target-prediction--skills-off-target-/ and loads when your prompt matches the skill's trigger.
When to invoke it: Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated.
Who uses this skill
The bio-genome-engineering-off-target-prediction Claude Code skill is built for researchers, data scientists, academics, and analysts working with complex data and scientific literature. It's part of ClaudSkills (also referred to as Claude Skills or Claude Code Skills) — the open community-curated registry of 93,000+ SKILL.md files for Anthropic's Claude Code agent and the wider Claude ecosystem (Claude API, Claude Agent SDK).
Or just download SKILL.md directly and drop it into ~/.claude/skills/bio-genome-engineering-off-target-prediction--skills-off-target-/. Claude Code auto-discovers it on next session.
Skills live at ~/.claude/skills/bio-genome-engineering-off-target-prediction--skills-off-target-/SKILL.md on macOS/Linux, or %USERPROFILE%\.claude\skills\bio-genome-engineering-off-target-prediction--skills-off-target-\SKILL.md on Windows. See the full install guide for step-by-step instructions.
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How do I install the bio-genome-engineering-off-target-prediction Claude Code skill?
Install via the ClaudSkills desktop app (one click) or copy SKILL.md from the source repository to ~/.claude/skills/bio-genome-engineering-off-target-prediction--skills-off-target-/SKILL.md and restart Claude Code. Both flows are detailed at claudskills.com/install/.
What does the bio-genome-engineering-off-target-prediction skill do?
Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.
Is this skill free to install?
Yes. ClaudSkills is an open registry — every skill keeps its source repository's license, and manual install via copy is free. ClaudSkills Pro ($9/mo, $79/yr, or $149 one-time) adds one-click install via the desktop app and a multi-signal Quality Score.
When should I use the bio-genome-engineering-off-target-prediction skill?
Use bio-genome-engineering-off-target-prediction when your Claude Code task falls under the Science & Research category — specifically in the biology medicine area. Claude Code auto-discovers installed skills and invokes the right one based on the task description, so you can also ask Claude directly (e.g. "use bio-genome-engineering-off-target-prediction" or describe the task and let Claude pick). Browse related skills at /category/science/.
What is a Claude Code skill and how does the bio-genome-engineering-off-target-prediction skill fit in?
A Claude Code skill is a SKILL.md file that lives under ~/.claude/skills/<name>/ and tells the Claude Code CLI agent how to perform a specific task (instructions, prompts, allowed tools). Skills are auto-discovered at session start. bio-genome-engineering-off-target-prediction is one of 67,000+ skills indexed in the open ClaudSkills catalog, classified under the Science & Research category. Learn more at /learn/what-is-a-claude-skill/.
@misc{bio-genome-engineering-off-target-prediction--skills-off-target--2026,
author = {bg-szy},
title = {bio-genome-engineering-off-target-prediction [Claude Code skill]},
year = {2026},
publisher = {ClaudSkills},
url = {https://claudskills.com/skills/bio-genome-engineering-off-target-prediction--skills-off-target-/}
}
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Claude™ is a trademark of Anthropic PBC. ClaudSkills (also referred to as Claude Skills or Claude Code Skills Catalog) is an independent community-curated registry of SKILL.md files, not affiliated with, endorsed by, or sponsored by Anthropic.
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