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Bio Long Read Splicing

Category: Content  ·  Sub-category: content-misc  ·  Last updated:
Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

About this skill (catalog notes)

Bio Long Read Splicing includes pricing or quota commentary; 8 code blocks for direct copy-paste. At roughly 3,047 words the SKILL.md is on the longer end of the catalog distribution.

Source
bg-szy.github.io/TOP-SKILLS
Original author
bg-szy
Indexed lastmod
Catalog position
Content · content-misc
Indexed related skills
10

How Bio Long Read Splicing fits the catalog

Bio Long Read Splicing sits in the Content category under the content-misc sub-topic in the ClaudSkills catalog. There are 10 related skills indexed alongside it; comparing a few before installing usually reveals which fits your workflow best.

These notes are auto-generated from features detected in the SKILL.md file and from this catalog's structure — they aren't part of the source repository.

What this skill does

Bio Long Read Splicing is a community-contributed Claude Code skill in the content-misc sub-category. It ships as a SKILL.md file that Claude Code auto-discovers under ~/.claude/skills/bio-long-read-splicing/ and loads when your prompt matches the skill's trigger.

When to invoke it: Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

Who uses this skill

The Bio Long Read Splicing Claude Code skill is built for content creators, marketers, copywriters, SEO professionals, and editorial teams. It's part of ClaudSkills (also referred to as Claude Skills or Claude Code Skills) — the open community-curated registry of 116,000+ SKILL.md files for Anthropic's Claude Code agent and the wider Claude ecosystem (Claude API, Claude Agent SDK).

How to install

Free

Manual install (2 steps)

mkdir -p ~/.claude/skills/bio-long-read-splicing
curl -L https://claudskills.com/skills/bio-long-read-splicing/SKILL.md \
  -o ~/.claude/skills/bio-long-read-splicing/SKILL.md

Or just download SKILL.md directly and drop it into ~/.claude/skills/bio-long-read-splicing/. Claude Code auto-discovers it on next session.

Skills live at ~/.claude/skills/bio-long-read-splicing/SKILL.md on macOS/Linux, or %USERPROFILE%\.claude\skills\bio-long-read-splicing\SKILL.md on Windows. See the full install guide for step-by-step instructions.

Telegram

📱 Install from your phone or desktop Telegram

Open @claudskills_bot on Telegram, tap Open Desktop App, and the desktop app installs this skill for you. Or share the bot link with a colleague — they get the same one-tap install. Learn more →

Pro

One-click install via the desktop app

The ClaudSkills desktop app installs any skill directly into ~/.claude/skills/ with one click — no terminal required. Pro starts at $9/mo or $149 lifetime.

Pro

For the full experience including quality scoring and one-click install features for each skill — upgrade to Pro.

Frequently asked questions

How do I install the Bio Long Read Splicing Claude Code skill?
Install via the ClaudSkills desktop app (one click) or copy SKILL.md from the source repository to ~/.claude/skills/bio-long-read-splicing/SKILL.md and restart Claude Code. Both flows are detailed at claudskills.com/install/.
What does the Bio Long Read Splicing skill do?
Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.
Is this skill free to install?
Yes. ClaudSkills is an open registry — every skill keeps its source repository's license, and manual install via copy is free. ClaudSkills Pro ($9/mo, $79/yr, or $149 one-time) adds one-click install via the desktop app and a multi-signal Quality Score.
When should I use the Bio Long Read Splicing skill?
Use Bio Long Read Splicing when your Claude Code task falls under the Content category — specifically in the content misc area. Claude Code auto-discovers installed skills and invokes the right one based on the task description, so you can also ask Claude directly (e.g. "use Bio Long Read Splicing" or describe the task and let Claude pick). Browse related skills at /category/content/.
What is a Claude Code skill and how does the Bio Long Read Splicing skill fit in?
A Claude Code skill is a SKILL.md file that lives under ~/.claude/skills/<name>/ and tells the Claude Code CLI agent how to perform a specific task (instructions, prompts, allowed tools). Skills are auto-discovered at session start. Bio Long Read Splicing is one of 67,000+ skills indexed in the open ClaudSkills catalog, classified under the Content category. Learn more at /learn/what-is-a-claude-skill/.

Attribution & license

Cite this skill

If you reference this skill in a blog post, paper, or documentation, you can cite it as:

APA
bg-szy. (2026). Bio Long Read Splicing [Claude Code skill]. ClaudSkills. https://claudskills.com/skills/bio-long-read-splicing/
BibTeX
@misc{bio-long-read-splicing-2026,
  author    = {bg-szy},
  title     = {Bio Long Read Splicing [Claude Code skill]},
  year      = {2026},
  publisher = {ClaudSkills},
  url       = {https://claudskills.com/skills/bio-long-read-splicing/}
}

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