Sgrna Design Guide

Quality score: 70/100 · Category: General · Sub-category: design-creative

Decision guide for finding/designing sgRNAs via three tiers: (1) validated from Addgene/literature, (2) pre-computed from Broad CRISPick, (3) de novo via CRISPOR/Benchling as last resort. Covers PAM rules (SpCas9, SaCas9, AsCas12a, enAsCas12a), quality metrics, and targeting rules for knockout, CRISPRa/i, base and prime editing. Use when planning CRISPR and unsure which sgRNA source to pick.

What this skill does

Sgrna Design Guide is a production-ready Claude Code skill (quality score 70/100) in the design-creative sub-category. It ships as a SKILL.md file that Claude Code auto-discovers under ~/.claude/skills/sgrna-design-guide/ and loads when your prompt matches the skill's trigger.

When to invoke it: Use when planning CRISPR and unsure which sgRNA source to pick.

Who uses this skill

The Sgrna Design Guide skill is built for Claude Code users and developers across all disciplines looking for general-purpose AI assistance. It is part of the open ClaudSkills registry, a community-curated catalog of 15,000+ capabilities you can install for Claude Code — the Claude CLI agent.

How to install

Free

Manual install (2 steps)

mkdir -p ~/.claude/skills/sgrna-design-guide
curl -L https://claudskills.com/skills/sgrna-design-guide/SKILL.md \
  -o ~/.claude/skills/sgrna-design-guide/SKILL.md

Or just download SKILL.md directly and drop it into ~/.claude/skills/sgrna-design-guide/. Claude Code auto-discovers it on next session.

Skills live at ~/.claude/skills/sgrna-design-guide/SKILL.md on macOS/Linux, or %USERPROFILE%\.claude\skills\sgrna-design-guide\SKILL.md on Windows. See the full install guide for step-by-step instructions.

Pro

One-click install via the desktop app

The ClaudSkills desktop app installs any skill directly into ~/.claude/skills/ with one click — no terminal required. Pro starts at $9/mo or $149 lifetime.

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Part of ClaudSkills — the open registry for Claude Code skills. · What's New · Install guide · About · llms.txt

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