Deep learning-based variant calling from long reads using Clair3 for SNPs and small indels. Use when calling germline variants from ONT or PacBio alignments, particularly — from…
Spectral similarity and compound identification for metabolomics. Use for comparing mass spectra, computing similarity scores (cosine, modified cosine), and identifying u — from…
Executive leadership guidance for strategic decision-making, organizational development, and stakeholder management.
When the user wants to understand or optimize for SERP feature types (PAA, sitelinks, rich results, AI Overviews).
Identify recurrent and driver copy number alterations across a tumor cohort with GISTIC2 (G-score, Ziggurat deconstruction, focal vs broad/arm-level analysis, q-values from…
Species abundance estimation using Bracken with Kraken2 output. Redistributes reads from higher taxonomic levels to species for more accurate estimates.
Vue 3 debugging and error handling for runtime errors, warnings, async failures, and SSR/hydration issues. Use when diagnosing or fixing Vue issues. — from bg-szy/TOP-SKILLS
Use when analyzing binary structure, disassembling code, or decompiling functions. Deep static analysis via radare2 (r2) and Ghidra headless - function enumeration, cross — from…
When the user wants to create, optimize, or audit solutions pages. Also use when the user mentions "solutions," "solutions page," "by industry," "industry solutions," "by company…
Orchestrates an end-to-end de novo genome assembly project, routing each step to the right genome-assembly skill rather than restating it.
Extract methylation calls from Bismark BAM files using bismark_methylation_extractor. Generates per-cytosine reports for CpG, CHG, and CHH contexts.
Full-featured computational pathology toolkit. Use for advanced WSI analysis including multiplexed immunofluorescence (CODEX, Vectra), nucleus segmentation, tissue graph — from…
Data-independent acquisition (DIA) proteomics analysis with DIA-NN and other tools. Use when analyzing DIA mass spectrometry data with library-free or library-based workf — from…
Create production-ready FastAPI projects with async patterns, dependency injection, and comprehensive error handling.
Gene set enrichment analysis with correct geneset format handling. Critical guidance for loading pathway databases and running enrichment in OmicVerse. — from bg-szy/TOP-SKILLS
Searches molecular libraries for substructure matches using SMARTS patterns with RDKit. Filters compounds by pharmacophore features, functional groups, or scaffold matche — from…
Phylogenetic tree toolkit (ETE). Tree manipulation (Newick/NHX), evolutionary event detection, orthology/paralogy, NCBI taxonomy, visualization (PDF/SVG), for phylogenomi — from…
Implement PCI DSS compliance requirements for secure handling of payment card data and payment systems.
Analyzes spatial proteomics data from CODEX, IMC, and MIBI platforms including cell segmentation and protein colocalization.
Draw and export phylogenetic trees using Biopython Bio.Phylo with matplotlib and modern alternatives.
Latch platform for bioinformatics workflows. Build pipelines with Latch SDK, @workflow/@task decorators, deploy serverless workflows, LatchFile/LatchDir, Nextflow/Snakema — from…
Use when implementing any feature or bugfix, before writing implementation code - write the test first, watch it fail, write minimal code to pass; ensures tests actually verify…
When the user wants to design, optimize, or audit newsletter signup forms. Also use when the user mentions "newsletter," "email signup," "subscribe form," "email capture," "lead…
Automated code review with best practices, security checks, and quality standards.
Detect single-nucleotide crosslink (CL) sites in CLIP-seq data using truncation patterns (iCLIP/eCLIP CITS), crosslink-induced mutations (HITS-CLIP CIMS deletions, PAR-CLIP…
Analyze TF motif accessibility variability across samples or single cells using chromVAR. Use when identifying TF motifs whose accessibility correlates with conditions, computing…
Pythonic wrapper around RDKit with simplified interface and sensible defaults. Preferred for standard drug discovery including SMILES parsing, standardization, descriptor — from…
Designs genomics experiments so technical nuisance variation (batch, lane, plate, flow cell, operator, reagent lot, processing day) is balanced against the biological variable of…
Generates comprehensive drug research reports with compound disambiguation, evidence grading, and mandatory completeness sections.
Post-translational modification analysis including phosphorylation, acetylation, and ubiquitination. Covers site localization, motif analysis, and quantitative PTM analys — from…
Develop AI-powered applications using Genkit in Node.js/TypeScript. Use when the user asks about Genkit, AI agents, flows, or tools in JavaScript/TypeScript, or when enco — from…
End-to-end alternative splicing analysis from FASTQ to differential splicing results for short-read bulk RNA-seq.
Creates new Claude Code skills following best practices. Guides through skill structure, naming, descriptions, and progressive disclosure.
Guide users through creating Agent Skills for Claude Code. Use when the user wants to create, write, author, or design a new Skill, or needs help with SKILL.md files, fro — from…
When the user wants to create TikTok video captions, scripts, or optimize for TikTok. Also use when the user mentions "TikTok post," "TikTok caption," "TikTok video," "po — from…
Master Python asyncio, concurrent programming, and async/await patterns for high-performance applications.
Predict peptide-MHC class I and II binding affinity using MHCflurry and NetMHCpan neural network models. Identify potential T-cell epitopes from protein sequences.
Predict metagenome functional content from 16S rRNA marker gene data using PICRUSt2. Infer KEGG, MetaCyc, and EC abundances from ASV tables.
Implement proven backend architecture patterns including Clean Architecture, Hexagonal Architecture, and Domain-Driven Design.
Preprocess small RNA sequencing data with adapter trimming and size selection optimized for miRNA, piRNA, and other small RNAs.
When the user wants to create, optimize, or audit contact page and forms. Also use when the user mentions "contact page," "contact form," "get in touch," "support form," "contact…
Build publication-quality figures in R with ggplot2 using the grammar of graphics (data + aesthetics + geometries + scales + facets + themes) with CVD-safe palettes, cairo_pdf…
Deep learning-based variant calling with Google DeepVariant. Provides high accuracy for germline SNPs and indels from Illumina, PacBio, and ONT data.
Visualize enrichment results using enrichplot package functions. Use when creating publication-quality figures from clusterProfiler results.
When the user wants to implement IndexNow, notify search engines of new/updated URLs, or speed up Bing indexing.
Gene Set Enrichment Analysis using clusterProfiler gseGO and gseKEGG. Use when analyzing ranked gene lists to find coordinated expression changes in gene sets without arb — from…
When the user wants to plan cold start, get first users, or launch a new product with zero traction. Also use when the user mentions "cold start," "cold start problem," "first…
Design, organize, and manage Helm charts for templating and packaging Kubernetes applications with reusable configurations.
PowerPoint (.pptx) manipulation via MCP server. Use for creating slides, formatting presentations, managing placeholders, adding images, applying templates, or extracting text…
Extracts, filters, annotates, and exports differential expression results from DESeq2 or edgeR with proper handling of padj=NA (independent filtering, Cook's outliers, all-zero),…
Search and retrieve clinical practice guidelines across 12+ authoritative sources including NICE, WHO, ADA, AHA/ACC, NCCN, SIGN, CPIC, CMA, CTFPHC, GIN, MAGICapp, PubMed,…
Comprehensive security engineering skill for application security, penetration testing, security architecture, and compliance auditing.
Master prompt engineering for AI models: LLMs, image generators, video models. Techniques: chain-of-thought, few-shot, system prompts, negative prompts.
Constructs and validates polygenic risk scores using LDpred2-auto, SBayesRC, MegaPRS, PRS-CS, PROSPER, MUSSEL, BridgePRS, JointPRS, PRSmix, or PGS Catalog Calculator with…
Web browser game development principles. Framework selection, WebGPU, optimization, PWA. — from bg-szy/TOP-SKILLS
When the user wants to create Reddit post copy, comments, or optimize for Reddit. Also use when the user mentions "Reddit post," "subreddit," "r/," "Reddit marketing," "p — from…
Import transcript-level quantifications from Salmon/kallisto into R for gene-level analysis with DESeq2/edgeR using tximport or tximeta.
Quality control for long-read sequencing data using NanoPlot, NanoStat, and chopper. Generate QC reports, filter reads by length and quality, and visualize read character — from…
When the user wants to add or optimize tab or accordion components for content organization. Also use when the user mentions "tab component," "accordion," "expandable content,"…
Creates reproducible Jupyter notebooks for bioinformatics analysis with parameterization using papermill.