Design and validate Type IIS scarless DNA assembly (Golden Gate, MoClo) using Biopython Bio.Restriction.
Validate documentation before merging - check completeness, broken links, code example accuracy, and factual correctness.
Automate Twitter/X with posting, engagement, and user management via inference.sh CLI. Apps: x/post-tweet, x/post-create (with media), x/post-like, x/post-retweet, x/dm-s — from…
Use when receiving code review feedback, before implementing suggestions, especially if feedback seems unclear or technically questionable - requires technical rigor and — from…
Analyze metabolite-mediated cell-cell communication using MeboCost for metabolic signaling inference between cell types.
Map identified threats to appropriate security controls and mitigations. Use when prioritizing security investments, creating remediation plans, or validating control eff — from…
Automated cell type annotation using reference-based methods including CellTypist, scPred, SingleR, and Azimuth for consistent, reproducible cell labeling.
Implement Command Query Responsibility Segregation for scalable architectures. Use when separating read and write models, optimizing query performance, or building event- — from…
Creates unique, production-grade frontend interfaces with exceptional design quality. Use when user asks to build web components, pages, materials, posters, or applications (e.g.,…
Neuropixels neural recording analysis. Load SpikeGLX/OpenEphys data, preprocess, motion correction, Kilosort4 spike sorting, quality metrics, Allen/IBL curation, AI-assis — from…
Implement state management patterns for frontend applications. Use when managing global state, handling complex data flows, or coordinating state across components.
Analyze images using GPT-4o Vision through the verging.ai proxy API, supporting both image URL (JSON) and file upload (multipart) modes.
Creative research ideation and exploration. Use for open-ended brainstorming sessions, exploring interdisciplinary connections, challenging assumptions, or identifying re — from…
High-performance toolkit for genomic interval analysis in Rust with Python bindings. Use when working with genomic regions, BED files, coverage tracks, overlap detection — from…
Extract, filter, annotate, and export differential expression results from DESeq2 or edgeR. Use for identifying significant genes, applying multiple testing corrections — from…
Search arXiv preprint repository for papers in physics, mathematics, computer science, quantitative biology, and related fields — from bg-szy/TOP-SKILLS
Use when completing tasks, implementing major features, or before merging to verify work meets requirements — from bg-szy/TOP-SKILLS
Discover genes associated with diseases and traits using GWAS data from the GWAS Catalog (500,000+ associations) and Open Targets Genetics (L2G predictions).
Build reproducible bioinformatics pipelines with Snakemake using rules, wildcards, and automatic dependency resolution.
Run a pre-deploy browser audit of a live, preview, or local web page for accessibility, SEO, Lighthouse quality, and critical UX issues.
Comprehensive patient stratification for precision medicine by integrating genomic, clinical, and therapeutic data.
Use when executing implementation plans with independent tasks in the current session — from bg-szy/TOP-SKILLS
Protein quantification from mass spectrometry data including label-free (LFQ, intensity-based), isobaric labeling (TMT, iTRAQ), and metabolic labeling (SILAC) approaches — from…
Domain vocabulary and entity relationships for {project}. Ensures consistent terminology and model understanding. — from bg-szy/TOP-SKILLS
Quality metrics for IMC data including signal-to-noise, channel correlation, tissue integrity, and acquisition QC.
EU MDR 2017/745 regulation specialist and consultant for medical device requirement management. Provides comprehensive MDR compliance expertise, gap analysis, technical d — from…
Materials science toolkit. Crystal structures (CIF, POSCAR), phase diagrams, band structure, DOS, Materials Project integration, format conversion, for computational mate — from…
Drive an authorized repository change through a verified local loop: baseline, reproduce, implement, test, review, and report evidence.
Production-ready genomics and epigenomics data processing for BixBench questions. Handles methylation array analysis (CpG filtering, differential methylation, age-related CpG…
Combine vector and keyword search for improved retrieval. Use when implementing RAG systems, building search engines, or when neither approach alone provides sufficient r — from…
Request and process code reviews with proper context. Use after completing significant implementation work.
Intelligently deploys Azure OpenAI models to optimal regions by analyzing capacity across all available regions.
Generates 3D conformer ensembles using RDKit ETKDGv3 with knowledge-enhanced distance geometry, MMFF94/UFF force-field optimization, CREST + GFN2-xTB semi-empirical refinement,…
Spectral similarity and compound identification for metabolomics. Use for comparing mass spectra, computing similarity scores (cosine, modified cosine), and identifying u — from…
Analyze metabolomics data including metabolite identification, quantification, pathway analysis, and metabolic flux.
Relight a still image — change the lighting setup, color temperature, direction, or mood — on RunComfy via the `runcomfy` CLI.
Read biological sequence files (FASTA, FASTQ, GenBank, EMBL, ABI, SFF) using Biopython Bio.SeqIO. Use when parsing sequence files, iterating multi-sequence files, random — from…
Understand what context is, why it matters, and the anatomy of context in agent systems
Build genome-browser-style multi-track figures with pyGenomeTracks (config-driven), Gviz (R), and IGV batch screenshotting.
Reads, writes, and converts molecular file formats (SMILES, InChI, SDF V2000/V3000, MOL2, PDB, MMTF) using RDKit and Open Babel with rigorous handling of aromaticity perception,…
Transform rough ideas into detailed designs through structured dialogue. Use before implementation to refine requirements.
Master Rust async programming with Tokio, async traits, error handling, and concurrent patterns. Use when building async Rust applications, implementing concurrent system — from…
End-to-end clinical trial analysis workflow from CDISC SDTM/ADaM loading through ICH E9(R1) estimand-driven primary analysis to CONSORT 2025 regulatory-compliant reporting.
Deep learning-based variant calling with Google DeepVariant. Provides high accuracy for germline SNPs and indels from Illumina, PacBio, and ONT data.
Preprocess ribosome profiling data including adapter trimming, size selection, rRNA removal, and alignment.
Analyzes spatial proteomics data from CODEX, IMC, and MIBI platforms including cell segmentation and protein colocalization.
Guidance for distinctive, intentional visual design when building new UI or reshaping an existing one.
Extracts and assigns COSMIC v3.4 mutational signatures (84 SBS / 11 DBS / 18 ID / 24 CN / 16 SV) from somatic VCFs using SigProfilerSuite, MutationalPatterns, MuSiCal mvNMF,…
Predict B-cell and T-cell epitopes using BepiPred, IEDB tools, and structure-based methods for vaccine and antibody design. Identify immunogenic regions in antigens.
Analyzes isoform switching events and functional consequences using IsoformSwitchAnalyzeR. Predicts protein domain changes, NMD sensitivity, ORF alterations, and coding potential…
Estimate bivariate genetic correlation (rg) between traits from GWAS summary statistics or individual-level genotypes using cross-trait LDSC, HDL, LAVA, rho-HESS, GREML-bivariate,…
Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignme — from…
Analyze multi-modal single-cell data (CITE-seq, Multiome, spatial). Use when working with data that measures multiple modalities per cell like RNA + protein or RNA + ATAC — from…
Analyzes your recent Claude Code chat history to identify coding patterns, development gaps, and areas for improvement, curates relevant learning resources from HackerNew — from…
Genomic file toolkit. Read/write SAM/BAM/CRAM alignments, VCF/BCF variants, FASTA/FASTQ sequences, extract regions, calculate coverage, for NGS data processing pipelines.
Analyze Reddit threads for sentiment, consensus opinions, top arguments, and discussion patterns. Use this when users want to understand Reddit community opinions, analyz — from…
Execute database migrations across ORMs and platforms with zero-downtime strategies, data transformation, and rollback procedures.
Detect horizontal gene transfer (HGT / LGT) using compositional methods (GC%, codon usage, tetranucleotide z-scores via SIGI-HMM, AlienHunter, IslandViewer 4, IslandPath- — from…
Guide for creating high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools.
Differentially methylated region (DMR) detection using methylKit tiles, bsseq BSmooth, and DMRcate. Use when identifying contiguous genomic regions with methylation diffe — from…