Trim multiple sequence alignments using ClipKIT, trimAl, BMGE, Divvier, or HMMcleaner with mode selection guidance per downstream goal.
Read, write, and convert multiple sequence alignment files using Biopython Bio.AlignIO. Supports Clustal, PHYLIP, Stockholm, FASTA, Nexus, and other alignment formats for — from…
Comprehensive software architecture skill for designing scalable, maintainable systems using ReactJS, NextJS, NodeJS, Express, React Native, Swift, Kotlin, Flutter, Postg — from…
Comprehensive variant filtering including GATK VQSR, hard filters, bcftools expressions, and quality metric interpretation for SNPs and indels.
When the user wants to create, optimize, or validate URL slugs for content pages. Also use when the user mentions "URL slug," "URL path," "blog URL," "article URL," "short URL,"…
When the user wants to choose an SEO-friendly domain for a new site—brand vs keyword domain, TLD selection, or domain best practices.
Spawn an AI coding agent in a new terminal (Claude, Codex, Gemini, Cursor, OpenCode, Copilot). Defaults to Claude Code if unspecified. — from bg-szy/TOP-SKILLS
Supervised and unsupervised multi-omics integration with mixOmics. Includes sPLS for pairwise integration and DIABLO for multi-block discriminant analysis.
Type-driven design patterns in Rust - typestate, newtype, builder pattern, and compile-time guarantees — from bg-szy/TOP-SKILLS
Master the uv package manager for fast Python dependency management, virtual environments, and modern Python project workflows.
When the user wants to plan or execute a rebrand—domain change, 301 redirects, migration, or announcement.
Use when starting feature work that needs isolation from current workspace or before executing implementation plans - creates isolated git worktrees with smart directory — from…
Domain vocabulary and entity relationships for {project}. Ensures consistent terminology and model understanding. — from bg-szy/TOP-SKILLS
Quality control and assessment for proteomics data. Use when evaluating proteomics data quality before downstream analysis.
Add p-value brackets, significance asterisks, and effect-size annotations to distribution plots using ggpubr, ggsignif, and statannotations with correct test selection (parametric…
Bayesian modeling with PyMC. Build hierarchical models, MCMC (NUTS), variational inference, LOO/WAIC comparison, posterior checks, for probabilistic programming and infer — from…
Identify and counteract cognitive biases in medical decision-making through systematic error analysis and contextual algorithm application.
Protein grouping and inference from peptide identifications. Use when resolving protein ambiguity from shared peptides.
When the user wants open source strategy, OSS commercialization, or open source growth. Also use when the user mentions "open source strategy," "OSS strategy," "open source…
Visualize enrichment results using enrichplot package functions. Use when creating publication-quality figures from clusterProfiler results.
Convert files and office documents to Markdown. Supports PDF, DOCX, PPTX, XLSX, images (with OCR), audio (with transcription), HTML, CSV, JSON, XML, ZIP, YouTube URLs, EP — from…
Performs logistic regression for clinical trial outcomes (binary, ordinal, multinomial) with marginal-vs-conditional estimand reporting per FDA 2023 covariate adjustment guidance,…
Controls error rates across thousands of simultaneous tests in genomics discovery using false-discovery-rate methods (Benjamini-Hochberg 1995; Benjamini-Yekutieli 2001 for…
Vite 8.0.10 build tooling — HMR, fast builds, plugins, and optimized production assets. Use when configuring Vite, setting up React/Vue projects with Vite, or optimizing frontend…
Comprehensive SecOps skill for application security, vulnerability management, compliance, and secure development practices.
Reactome pathway enrichment using ReactomePA package. Use when analyzing gene lists against Reactome's curated peer-reviewed pathway database.
Map metabolites to biological pathways using KEGG, Reactome, and MetaboAnalyst. Perform pathway enrichment and topology analysis.
Convert Figma or Pencil designs into production-ready frontend code using Feature-Sliced Design and Tailwind CSS v4.
Extract text and tables from PDF files, fill forms, merge documents. Use when working with PDF files or when the user mentions PDFs, forms, or document extraction.
Design pricing, packaging, and monetization strategies based on value, customer willingness to pay, and growth objectives. — from bg-szy/TOP-SKILLS
Statistical testing for differentially abundant proteins between conditions. Covers preprocessing (log2 transformation, normalization), limma and DEqMS workflows with emp — from…
Query dbSNP for rsID lookups, variant annotations, and cross-references to other databases. Use when mapping between rsIDs and genomic coordinates or retrieving basic var — from…
Process Hi-C read pairs using pairtools. Parse alignments, filter duplicates, classify pairs, and generate contact statistics from Hi-C sequencing data.
Detect and quantify translated ORFs from Ribo-seq data including uORFs and novel ORFs using RiboCode and ORFquant.
Access NCBI GEO for gene expression/genomics data. Search/download microarray and RNA-seq datasets (GSE, GSM, GPL), retrieve SOFT/Matrix files, for transcriptomics and ex — from…
Toolkit for interacting with and testing local web applications using Playwright. Supports verifying frontend functionality, debugging UI behavior, capturing browser scre — from…
Generates standardized quality control reports by aggregating metrics from FastQC, alignment, and other tools using MultiQC.
WordPress performance optimization - Core Web Vitals, image/video compression, caching, asset optimization, and speed testing.
Extend or continue an existing video clip on RunComfy via the `runcomfy` CLI. Routes to Google Veo 3-1's `extend-video` and `fast/extend-video` endpoints — pick the source video…
Perform code reviews following Sentry engineering practices. Use when reviewing pull requests, examining code changes, or providing feedback on code quality.
Calls ChIP-seq peaks with MACS3, MACS2, HOMER, or SPP across narrow (TF) and broad (histone) modes. Handles input control matching, fragment-size modeling vs --nomodel, effective…
Comprehensive code review skill for TypeScript, JavaScript, Python, Swift, Kotlin, Go. Includes automated code analysis, best practice checking, security scanning, and re — from…
Build circular genome visualizations using circlize (R), pyCirclize (Python), or Circos (Perl CLI) with ideogram tracks, multi-data tracks (scatter, histogram, heatmap),…
Selects informative features for biomarker discovery using Boruta all-relevant selection, mRMR minimum redundancy, and LASSO regularization.
Protein quantification from mass spectrometry data including label-free (LFQ, intensity-based), isobaric labeling (TMT, iTRAQ), and metabolic labeling (SILAC) approaches — from…
Create AI agents and workflows using Microsoft Agent Framework SDK. Supports single-agent and multi-agent workflow patterns.
Master guide for using Claude Code effectively. Includes configuration templates, prompting strategies "Thinking" keywords, debugging techniques, and best practices for i — from…
Performs retrosynthetic planning using AiZynthFinder (MCTS, template-based), Chemformer (template-free transformer), ASKCOS, and emerging RetroSynFormer with explicit handling of…
Create commit messages following Sentry conventions. Use when committing code changes, writing commit messages, or formatting git history.
Automate changelog generation from commits, PRs, and releases following Keep a Changelog format. Use when setting up release workflows, generating release notes, or stand — from…
Senior Quality Manager Responsible Person (QMR) for HealthTech and MedTech companies. Provides overall quality system responsibility, regulatory compliance oversight, man — from…
Guided statistical analysis with test selection and reporting. Use when you need help choosing appropriate tests for your data, assumption checking, power analysis, and A — from…
Document toolkit (.docx). Create/edit documents, tracked changes, comments, formatting preservation, text extraction, for professional document processing.
When the user wants to optimize the title tag, page title, or SERP title. Also use when the user mentions "title tag," "meta title," "page title," "SEO title," "SERP title,"…
Tests associations between categorical variables in clinical data using chi-square, Fisher's exact, Boschloo, Cochran-Mantel-Haenszel, and modern McNemar variants with calibrated…
FHIR API development guide for building healthcare endpoints. Use when: (1) Creating FHIR REST endpoints (Patient, Observation, Encounter, Condition, MedicationRequest), (2)…
Simplify and refine recently modified code for clarity and consistency. Use after writing code to improve readability without changing functionality. — from bg-szy/TOP-SKILLS
Quality control and normalization for metabolomics data. Covers QC-based correction, batch effect removal, and data transformation methods.
Generate AI images with GPT-Image-2, FLUX, Gemini, Grok, Seedream, Reve and 50+ models via inference.sh CLI.
Analyzes meeting transcripts and recordings to uncover behavioral patterns, communication insights, and actionable feedback.