Use when completing tasks, implementing major features, or before merging to verify work meets requirements - dispatches code-reviewer subagent to review implementation a — from…
Use when creating new skills, editing existing skills, or verifying skills work before deployment — from bg-szy/TOP-SKILLS
Query ClinVar for variant pathogenicity classifications, review status, and disease associations via REST API or local VCF.
Manage major dependency version upgrades with compatibility analysis, staged rollout, and comprehensive testing.
Load spatial transcriptomics data from Visium, Xenium, MERFISH, Slide-seq, and other platforms using Squidpy and SpatialData.
Orchestrates the end-to-end bulk short-read alternative-splicing pipeline from FASTQ to differential splicing, chaining fastp QC, cohort-consistent STAR 2-pass alignment (one…
Build enhancer-driven gene regulatory networks (eGRNs) by integrating single-cell RNA-seq and ATAC-seq using SCENIC+, CellOracle base GRNs, Pando, FigR, DIRECT-NET, TRIPO — from…
Automate Twitter/X with posting, engagement, and user management via inference.sh CLI. Apps: x/post-tweet, x/post-create (with media), x/post-like, x/post-retweet, x/dm-s — from…
Web browser game development principles. Framework selection, WebGPU, optimization, PWA. — from bg-szy/TOP-SKILLS
Run the Gemini file processor on any folder — extracts content from PDF, PPTX, XLSX, DOCX, CSV, JSON, and any text format, then generates Obsidian-ready summaries.
Handles batch effects in bulk RNA-seq via design-matrix inclusion (the correct path for DE), ComBat/ComBat-seq for visualization, SVA for unknown latent factors, RUVSeq for…
Spillover compensation and data transformation for flow cytometry. Covers compensation matrix calculation, application, and biexponential/arcsinh transforms.
Extract and analyze data from invoices, receipts, bank statements, and financial documents. Categorize expenses, track recurring charges, and generate expense reports.
Comprehensive backend development guide for Node.js/Express/TypeScript microservices. Use when creating routes, controllers, services, repositories, middleware, or workin — from…
ChIP-seq quality control metrics including FRiP (Fraction of Reads in Peaks), cross-correlation analysis (NSC/RSC), library complexity, and IDR (Irreproducibility Discove — from…
Create opinionated premium DESIGN.md guidance for Stitch, emphasizing calibrated typography, restrained color, layout discipline, motion, and anti-generic UI rules.
Comprehensive variant filtering including GATK VQSR, hard filters, bcftools expressions, and quality metric interpretation for SNPs and indels.
Quality control for flow, spectral, and mass cytometry - time-based anomaly cleaning (flowAI, flowCut, PeacoQC, flowClean), margin/boundary event removal, signal-drift detection,…
Create and optimize popups, modals, overlays, slide-ins, and banners to increase conversions without harming user experience or brand trust. — from bg-szy/TOP-SKILLS
Deep learning-based variant calling from long reads using Clair3 for SNPs and small indels. Use when calling germline variants from ONT or PacBio alignments, particularly — from…
Build, manage, and search spectral libraries for proteomics. Use when creating or working with spectral libraries for DIA analysis.
Discovers available Azure OpenAI model capacity across regions and projects. Analyzes quota limits, compares availability, and recommends optimal deployment locations bas — from…
Calls DNA methylation from Oxford Nanopore sequencing data using signal-level analysis. Use when detecting 5mC or 6mA modifications directly from nanopore reads without b — from…
Golang performance optimization patterns and methodology - if X bottleneck, then apply Y. Covers allocation reduction, CPU efficiency, memory layout, GC tuning, pooling — from…
Publish Markdown articles to X (Twitter) Articles editor with proper formatting. Use when user wants to publish a Markdown file/URL to X Articles, or mentions "publish to — from…
End-to-end outbreak investigation from pathogen isolates to transmission networks. Orchestrates MLST typing, AMR surveillance, phylodynamic dating, and transmission infer — from…
Use when completing tasks, implementing major features, or before merging to verify work meets requirements — from bg-szy/TOP-SKILLS
Create, refine, resume, publish, or query polished PersonWise courses from topics, text, PDF/PPTX/DOCX/Markdown/TXT documents, or reference images with the PersonWise CLI.
Orders and orients assembled contigs into chromosome-scale scaffolds from long-range linking data, inserting N-gap spacers (adds no sequence).
World-class ML engineering skill for productionizing ML models, MLOps, and building scalable ML systems.
Create high-converting, visually distinctive landing pages. Use when building marketing pages, product launches, SaaS homepages, or any single-page conversion-focused web — from…
A TikTok API alternative on fetcher.sh — pay-per-call in USDC via x402, or prepaid credits with a Bearer key, no login and no app review.
Access USPTO APIs for patent/trademark searches, examination history (PEDS), assignments, citations, office actions, TSDR, for IP analysis and prior art searches.
Jest testing patterns, factory functions, mocking strategies, and TDD workflow. Use when writing unit tests, creating test factories, or following TDD red-green-refactor — from…
Unsupervised clustering and cell-type identification for high-dimensional flow, spectral, and mass cytometry - FlowSOM, PhenoGraph, FlowSOM-via-CATALYST, with UMAP/tSNE f — from…
When the user wants to create, optimize, or audit migration guides for users switching from competitors.
Provide comprehensive clinical interpretation of somatic mutations in cancer. Given a gene symbol + variant (e.g., EGFR L858R, BRAF V600E) and optional cancer type, performs…
Chart selection and data visualization guidance for effective data communication. Use when: creating visualizations, choosing chart types, designing dashboards, or when user…
Smart dependency management for any language. Auto-detects project type, applies safe updates automatically, prompts for major versions, diagnoses and fixes dependency issues.
Picks random winners from lists, spreadsheets, or Google Sheets for giveaways, raffles, and contests. Ensures fair, unbiased selection with transparency. — from bg-szy/TOP-SKILLS
Render videos from React/Remotion component code via inference.sh. Pass TSX code, get MP4. Supports all Remotion APIs: useCurrentFrame, useVideoConfig, spring, interpolat — from…
Master creating MCP tools with type-safe parameters, automatic schema generation, and best practices — from bg-szy/TOP-SKILLS
Modify protein structures using Biopython Bio.PDB. Use when transforming coordinates, removing atoms or residues, adding new entities, modifying B-factors and occupancies — from…
Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Covers model selection, GPU acceleration, modified base detection, and qual — from…
Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published…
When the user wants to create, optimize, or audit media kit or press page. Also use when the user mentions "media kit," "press kit," "press page," "press resources," "brand…
When the user wants to create, optimize, or audit About page content. Also use when the user mentions "about page," "about us," "company story," "our team," "about section,"…
Generate AI images with FLUX, Gemini, Grok, Seedream, Reve and 50+ models via inference.sh CLI. Models: FLUX Dev LoRA, FLUX.2 Klein LoRA, Gemini 3 Pro Image, Grok Imagine — from…
Manage database schema changes with version control. Use when modifying DB schema, adding tables/columns, or setting up new projects.
Calculate polygenic risk scores using PRSice-2, LDpred2, or PRS-CS from GWAS summary statistics. Use when predicting disease risk from genome-wide genetic variants.
Analyze mass spectrometry proteomics data including protein quantification, differential expression, post-translational modifications (PTMs), and protein-protein interactions.
Still-to-video conversion guide: model selection, motion prompting, and camera movement. Covers Wan 2.5 i2v, Seedance, Fabric, Grok Video with when to use each.
When the user wants to create, optimize, or audit the main site homepage (primary entry page). Also use when the user mentions "homepage," "main page," "home page," "hero…
Detect structural variants from long-read alignments using Sniffles, cuteSV, and SVIM. Use when detecting deletions, insertions, inversions, translocations, or complex re — from…
Analyze BCR repertoires for somatic hypermutation, clonal lineages, and B cell phylogenetics using the Immcantation framework.
Build and interpret polygenic risk scores (PRS) for complex diseases using GWAS summary statistics. Calculates genetic risk profiles, interprets PRS percentiles, and assesses…
Cell segmentation from multiplexed tissue images. Covers deep learning (Cellpose, Mesmer) and classical approaches for nuclear and whole-cell segmentation.
Build a throwaway prototype to answer a design question. Use when the user wants to sanity-check whether a state model or logic feels right, or explore what a UI should l — from…
Use when you have a spec or requirements for a multi-step task, before touching code — from bg-szy/TOP-SKILLS
Calls m6A peaks from MeRIP-seq / m6A-seq paired IP-vs-input data using exomePeak2 (transcript-aware, GC-bias-corrected Poisson GLM; Liu 2022 *NAR Genom Bioinform* 4:lqac046),…